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Published by Cold Spring Harbor Laboratory in Cold Spring Harbor, N.Y .
Written in English


  • Nucleases.

Book details:

Edition Notes

Includes bibliographies and index.

Statementedited by Stuart M. Linn, Richard J. Roberts.
SeriesCold Spring Harbor monograph series ;, 14
ContributionsLinn, Stuart M., Roberts, Richard J.
LC ClassificationsQP609.N78 N8 1985
The Physical Object
Paginationviii, 390 p., [1] leaf of plates :
Number of Pages390
ID Numbers
Open LibraryOL3029135M
ISBN 100879691824
LC Control Number85009653

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I. Schildkraut, in Encyclopedia of Genetics, A nuclease is an enzyme that degrades nucleic acids by hydrolyzing the phosphodiester bond that joins the sugar residues. Nucleases are critical components to biological processes involving nucleic acids. Some nucleases are DNA specific (DNase), some are RNA specific (RNase), and some degrade both DNA and RNA. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related : Paperback. Nucleases that exist in serum or tissue can degrade naked nucleic acid therapeutics (antisense oligonucleotides, ribozymes, and RNAi). Nanotechnology has presented some solutions for resolving these problems. Nanovehicles can deliver nucleic acid through blood, protect the cargo from nucleases, opsonization and glomeral filtration, induce endocytosis of vehicle with its cargo, and finally. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.

Nuclease: Defintion. The suffix ase generally refers to enzymes. In this case, nuclease (pronounced nuk-lee-aize) is an enzyme that is responsible for breaking the bonds between nucleotides in. Nucleases. New York: Cold Spring Harbor Laboratory Press, (OCoLC) Material Type: Internet resource: Document Type: Book, Internet Resource: All Authors / Contributors: Stuart M Linn; R Stephen Lloyd; Richard J Roberts. Nuclease, any enzyme that cleaves nucleic acids. Nucleases, which belong to the class of enzymes called hydrolases, are usually specific in action, ribonucleases acting only upon ribonucleic acids (RNA) and deoxyribonucleases acting only upon deoxyribonucleic acids (DNA). Some enzymes having a. nuclease[′nüklē‚ās] (biochemistry) An enzyme that catalyzes the splitting of nucleic acids to nucleotides, nucleosides, or the components of the latter. Nuclease a phosphodiesterase enzyme that splits nucleic acids into mononucleotides and oligonucleotides. Nucleases are widely distributed in the cells of microorganisms, plants, and animals.

Nucleases. [Stuart M Linn; Richard J Roberts;] Home. WorldCat Home About WorldCat Help. Search. Search for Library Items Search for Lists Search for Book, Internet Resource: All Authors / Contributors: Stuart M Linn; Richard J Roberts. Find more information about: ISBN: Molecular Biology of Nucleases - CRC Press Book Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Genome editing, or genome engineering, or gene editing, is a type of genetic engineering in which DNA is inserted, deleted, modified or replaced in the genome of a living organism. Unlike early genetic engineering techniques that randomly inserts genetic material into a host genome, genome editing targets the insertions to site specific locations. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for.